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Anisotropy T-format using MOS-200M and an additional PMS-250
New technical note - TN61
(for registered users - see here)
Pre-configured systems - Combining the highest performance and best prices.
For many years Bio-Logic has led the industry in providing the highest quality and best performing rapid kinetics systems. We are now taking the lead in providing the same quality and performance, at prices that are incredibly competitive for today's user.
We have assembled packages containing our most popular combinations of stopped flow mixers and optical systems, and set package prices that are significantly lower than the total component prices.
The stopped flow and optical systems are identical to those available separately and are completely compatible with all of the same options and accessories.
The package systems allow you to greatly increase your range of applications for a system and stay within your budget.
For example, you could order an SFM-300/S and MOS-200 separately. Or, for a slightly lower total price, you can now get the SFM-300/S + MOS-200 package, and add the freeze quench accessory, and also add the quench flow upgrade for the SFM-300.
If you are planning to get a system in the near future, contact us now for a current quote including one of these packages and the options or accessories important for your applications.
» Contact us
An review of state of the art stopped-flow measurements on synchrotron and neutron beam lines
Applications of stopped-flow in SAXS and SANS
Isabelle Grillo
Current Opinion in Colloid and Interface Science 14 (2009) 402-408
(SFM-300)
X-ray Head and Capillary holder
New technical note - TN60
(for registered users - see here)
Single wavelength kinetics experiment using MOS-200M/CD in CD mode
New technical note -
TN59
(for registered users - see
here)
New Bio-Kine32 version
V4.51 available for download to
registered users.
New section in "Selection of scientific articles using the Bio-Logic rapid kinetics
systems"
Stopped-flow (mT jump)
Frequently asked questions
Please take a look at our new FAQ section.
Our instruments in anaerobic conditions
(updated March 1st, 2007)
Our instruments have been used in several occasions in
anaerobic conditions.
For more information please click here
Infrared detection for our Stopped-Flow!
A Millisecond Infrared Stopped-Flow Apparatus
Jia Tang and Feng Gai Applied Spectroscopy, Vol. 60, Issue 12, pp.
1477-1481
(SFM-300 + FT-IR accessory)
More articles here
Titration of Cytochrome c using the titrator accessory
New technical note -
TN58
(for registered users - see
here)
Rapid Mixing Temperature Jump System
• Hot and cold jumps
• T-changes as high as several ten of degrees
• Low volume consumption
• Millisecond resolution
For more information please click here
See also our references using Stopped-flow
(mT jump)
Participation to the EMBO Practical Course
Bio-Logic was pleased to participate to
"Transient
Kinetics Methods Applied to Biological Macromolecules"
18th to 24th June 2006 at the Department of Biosciences, University of
Kent, UK
Designing double mixing quench-flow experiments
New technical note -
TN52
(for registered users - see
here)
Stopped-flow conductimetry
A new detection mode for Rapid-Kinetics
For more information please click
here (PDF file)
Rapid-Kinetics experiments down to -90C°
The cryo-stopped-flow assembly extends the
range of operation of the Bio-Logic stopped-flow instruments to far
sub-zero temperatures. It allows rapid kinetics experiments to be
performed down to -90°C." - Click
here (PDF
file)
for more information
Faster sampling rate for Bio-Kine32
Due to the constant increase in speed of the
Bio-Logic Stopped-Flow instruments, the data acquisition rate of
Bio-Kine32 in transient recording mode has been increased to 100 kHz (10
µs per data point). - Click here
for more information
Special mixing of viscous solutions
In our all our Stopped-Flow instruments,
syringe velocity is programmed and thus is independent of the solution
viscosity. Very asymmetric mixing such as pure glycerol against water
can therefore be achieved... - Click here
for more information
New specification for our SFM-300 & SFM-400
0.25 ms dead time with the new
microvolume accessory
Click here for more information
From Neutron Scattering & Synchrotron Radiation to Microgravity
experiments in parabolic flights
Bio-Logic instruments are today the N°1 choice
for experiments in the most stringent conditions.
- Click here
for information on Synchrotron Radiation / Neutron Scattering
experiments
- Click here
for information on Microgravity experiment
Improved and simplified control of
stepping motor with new software driver
A new stopped-flow software driver is now
available (MPS32 V2.02). In addition to the classical interface it now
offers an advanced mode that provides improved and simplified control of
stepping motor action resulting in high precision mixing ratio and
reproducibility. In addition, this new mode of control offers full users
assistance in the instrument usage. It is highly recommended that
SFM-300/400 and SFM-20 users download this last version of the
stopped-flow driver - click here for more
details.
Titrator and Peltier element controller
Because of its extreme
precision of volume delivery, the Bio-Logic stopped-flow instruments
feature the most accurate titrator of the market. By a simple software
choice and an easy hardware installation, any SFM-300/400 or SFM-20 can be
converted in a high precision two syringe titrator. The
titrator device also comes in a version that includes a Peltier Cell
temperature cycler. This Peltier/titrator system benefit from a full
integration with Spectrometer control software (Bio-Kine32). As such it
can easily be used in absorbance, fluorescence, fluorescence anisotropy
and circular dichoism.
New plungers and valves
The old styled Teflon plunger and the old
valves have been replaced by a new type of plunger with o-ring and a new
type of valve with o-rings. The result obtained is a perfect seal
whatever the temperature excursion is.
New hardstop valve
Small theoretical deadtime is one thing ! However
the quality of the real "stop" is more important: in many cases
"stop" artifacts prevent users from analyzing the first data
points of the Kinetics. We are now using a specially designed
"hardstop" valve for a quasi perfect stop.
New articles
- A Calibration Reaction for Rapid Freeze-Quench W-Band EPR
- Applications of stopped-flow in SAXS and SANS
- Binding kinetics of glucose and allosteric activators to human glucokinase reveal multiple conformational states
- Local structure of reaction intermediates probed by time-x-ray absorption near edge structure spectroscopy
- The mechanism of addition of pyridoxal 5´-phosphate to Escherichia
coli apo-serine hydroxymethyltransferase
- An allylic ketyl radical intermediate in clostridial amino-acid
fermentation
- Distinct Unfolding and Refolding Pathways of Ribonuclease A Revealed by
Heating and Cooling Temperature Jumps
- Micellization Kinetics of a Novel Multi-Responsive Double
Hydrophilic Diblock Copolymer Studied by Stopped-Flow pH and Temperature
Jump
- Cytochrome b5 Increases the Rate of Product Formation by Cytochrome P450
2B4 and Competes with Cytochrome P450 Reductase for a Binding Site on
Cytochrome P450 2B4
-
Key feature of the catalytic cycle of TNF-α converting enzyme involves
communication between distal protein sites and the enzyme catalytic core
- A semiquinone intermediate generated at the Qo site of the
cytochrome bc1 complex: Importance for the Q-cycle and superoxide
production
- Identification of Alpha-1 Acid Glycoprotein as a Lysophospholipid
Binding Protein: A Complementary Role to Albumin in the Scavenging of
Lysophosphatidylcholine
-
Biochemical Characterization and Ligand Binding Properties of
Neuroglobin, a Novel Member of the Globin Family
- Characterization of human D-amino acid oxidase
- Reduction of ferric haemoproteins by tetrahydropterins: a kinetic
study
-
A Millisecond Infrared Stopped-Flow Apparatus
-
Covalent heme attachment in Synechocystis hemoglobin is required to
prevent ferrous heme dissociation
-
Multistate reaction kinetics of 6-hydroxy-4-(dimethylamino)flavylium
driven by pH. A stopped-flow study
-
Specific Aquaporins Facilitate the Diffusion of Hydrogen Peroxide across
Membranes
-
Escherichia coli RecQ Is a Rapid, Efficient, and Monomeric Helicase
-
The Formin Homology 1 Domain Modulates the Actin Nucleation and Bundling
Activity of Arabidopsis FORMIN1
-
RecQ Helicase-catalyzed DNA Unwinding Detected by Fluorescence Resonance
Energy Transfer
-
Revisiting a proposed kinetic model for the reaction of cysteine and
hydrogen peroxide via cysteine sulfenic acid
-
Structural Characterization of Apomyoglobin Self-Associated Species in
Aqueous Buffer and Urea Solution
-
Structural Insights into the Functional Interaction of KChIP1 with Shal-Type
K+ Channels
-
In situ investigation of the oxidative addition in homogeneous Pd
catalysts by synchronised time resolved UV-Vis/EXAFS
-
Amyloid formation under physiological conditions proceeds via a
native-like folding intermediate
-
Polymerization of Anionic Wormlike Micelles
- pH-Induced Micellization Kinetics of ABC Triblock Copolymers
Measured by Stopped-Flow Light Scattering
- A Stopped-Flow Kinetic Study of the Assembly of Interpolymer
Complexes via Hydrogen-Bonding Interactions
- First Observation of Two-Stage Collapsing Kinetics of a Single
Synthetic Polymer Chain
- Mechanistic Information from Low-Temperature Rapid-Scan and NMR
Measurements on the Protonation and Subsequent Reductive Elimination
Reaction of a (Diimine)platinum(II) Dimethyl Complex
- Mechanism of a-oxoamine synthases: identification of the intermediate
Claisen product in the 8-amino-7-oxononanoate synthase reaction
- Probing the Kinetics of Membrane-Mediated Helix Folding
- Time-resolved scattering investigations of brome mosaic virus
microcrystals appearance
- Efficient Refolding of Aggregation-prone Citrate Synthase by Polyol
Osmolytes HOW WELL ARE PROTEIN FOLDING AND STABILITY ASPECTS COUPLED?*
- Ribose 2'-hydroxyl groups in the 5' strand of the acceptor arm of
P-site tRNA are not essential for EF-G catalyzed translocation
- The mechanism of GroEL/GroES folding/refolding of protein substrates
revisited
- Trifluoroethanol-Induced Unfolding of Concanavalin A: Equilibrium
and Time-Resolved Optical Spectroscopic Studies
- Tryptophan 243 affects interprotein contacts, cofactor binding and
stability in D-amino acid oxidase from Rhodotorula gracilis
- Structural and kinetics characterization of
the simplified SH3 domain FP1
- Stabilization of Native Protein fold by
intein-mediated covalent cyclization
- Computational redesign of protein-protein
interaction specificity
- Escherichia coli RecQ Is a Rapid, Efficient,
and Monomeric Helicase
- Evidence of Two Distinct Oxygen Complexes of
Reduced Endothelial Nitric Oxide Synthase
- Pore selectivity analysis of an
aquaglyceroporin by stopped-flow spectrophotometry on bacterial cell
suspensions.
- Probing the Reorganization of the Nicotinic
Acetylcholine Receptor during Desensitization by Time-Resolved
Covalent Labeling Using [3H]AC5, a Photoactivatable Agonist.
- RecQ Helicase-catalyzed DNA unwinding
detected by fluorescence resonance energy transfer
- The Heparin Binding Properties of Heparin
Cofactor II Suggest an Antithrombin-like Activation Mechanism
- Rapid mixing methods for exploring the
kinetics of protein folding
- Cooperative Sub-Millisecond Folding Kinetics
of Apomyoglobin pH 4 Intermediate
- Dynamics of structural transitions in
amphiphilic systems monitored by scattering techniques
- An advanced EPR stopped-flow apparatus based
on a dielectric ring resonator
- Stopped-flow and Mutational Analysis of Base
Flipping by the Escherichia coli Dam DNA-(adenine-
N6)-methyltransferase
- Steps towards Phage Display Libraries with an
extended amino acid repertoire
- DNA recognition by the Homing Endonuclease
PI-SceI involves a divalent metal ion cofactor induced conformational
change
- Apo-azurin folds via an intermediate
resembles the moten-globule
- Probing the influence on folding behavior of
structurally conserved core residues in P.Aerogonesa apo-azurin
- Acid-base behavior of organopalladium
complexes [Pd(CNN)R]BF4
- Two coupled photochromic systems of
3',4'-(methylendioxy)flavilium : kinetic and thermodynamic
characterisation
- Properties of some variants of human
beta2-microglobulin and amyloidogenesis
- Human Rhesus-associated glycoprotein mediates
facilitated transport of NH3 into red blood cells
- Roles of conserved P domain residues and Mg2+
in ATP binding in the ground and Ca2+ activated states of sarcoplasmic
reticulum Ca2+ - ATPase
- Glutamate-183 in the conserved TGES motif of
domain A of sarcoplasmic reticulum Ca2+ - ATPase assists in catalysis
of E2/E2P partial reactions
- Importance of transmembrane segment M1 of the
sarcoplasmic reticulum Ca2+-ATPase in Ca2+ occlusion and phosphoenzyme
processing
- Roles of Leu249, Lys252 and Leu253 in
membrane segment M3 of sarcoplasmic reticulum Ca2+-ATPase in control
of Ca2+ migration and Long-range intramolecular communication
- Dissection of the functionnal differences
between sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) 1 and 2
isoforms and characterization of darier disease (SERCA2)mutants by
steady-state and transient kinetic analysis
- Importance of conserved N-domain residues
thr441, Glu442, lys515, Arg560, Leu562 of sarcoplasmic reticulum
Ca2+-ATPase for MgATP binding and subsequent catalytic steps
- Importance of conserved Thr214 in domain A of
the Na+, K+ - ATPase for stabilization of the phosphoryl state complex
in E2P dephosphorylation
- Functional consequences of alterations of
Ile279, Ile283, Glu284, his285, Phe286, and His288 in the NH2-terminal
part of the transmembrane helix M3 of the Na+, K+ - ATPase M.
- Dissection of the functionnal differences
between sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) 1 and 3
isoforms by steady-state and transient kinetic analysis
- Importance of Glu282 in transmembrane segment
M3 of the of the Na+,K+-ATPase for control of the cation interaction
and conformational changes
- Importance of transmembrane segment M3 of the
sarcoplasmic reticulum Ca2+-ATPase for control of the gateway to the
Ca2+ sites
- Importance of Thr-353 of the conserved
phosphorylation loop of the sarcoplasmic reticulum Ca2+-ATPase in
MgATP binding and catalytic activity
- Mutational effects on conformational changes
of the dephospho-and phospho-forms of the Na+,K+-ATPase
- Importance of stalk segment S5 for
Intramolecular communication in the sarcoplasmic Ca2+-ATPase
- Revisiting the role of H+ in chmotactic
signaling of sperm
- Facile Variation of Reagent Concentrations in
Rapid Quench Enzymology.
- The signal flow and motor response controling
chemotaxis of sea urchin sperm.
- Structural reorganization of the Acetylcholine binding site of the Torpedo
nicotinic receptor as revealed by dynamic photoaffinity labeling
- Enzymatic mechanism of RNA translocation in
dsRNA bacteriophages
- Amino Acids of the Bacterial Toxin SopE
Involved in G Nucleotide Exchange on Cdc42
- The influence of hinge region residue Glu381
on antithrombin allostery and metastability.
- Catalytic Mechanism of the Haloalkane
Dehalogenase LinB from Sphingomonas paucimobilis UT26.
- Kinetic Analysis of R67 Dihydrofolate
Reductase Folding: From the Unfolded Monomer to the Native Tetramer
- Kinetic Characterization of the pH-Dependent
Oligomerization of R67 Dihydrofolate Reductase
- Role of individual disulfide bonds in hen
lysozyme early folding steps
- Immunochemical pulsed-labeling
characterization of intermediates during hen lysozyme oxidative
folding
- How does ZrO2/surfactant mesophase nucleate?
Formation mechanism
- Formation and growth of anionic vesicles
followed by small angle neutron scattering
- The complex between a four-way DNA junction
and T7 endonuclease 1
- Mapping the Cytochrome c Folding Landscape
- Surface Expansion Is Independent of and
Occurs Faster than Core Solvation during the Unfolding of Barstar
- DNA: REPLICATION REPAIR AND RECOMBINATION:
Kinetic Mechanism for the Formation of the Presynaptic Complex of the
Bacterial Recombinase RecA
- Characterization of Single-Tryptophan Mutants
of Histidine-Containing Phosphocarrier Protein: Evidence for Local
Rearrangements during Folding from High Concentrations of Denaturant
- Phosphorylation of the WASP-VCA Domain
Increases Its Affinity for the Arp2/3 Complex and Enhances Actin
Polymerization by WASP
- Metal ions bound at the active site of the
junction-resolving enzyme T7 endonuclease I.
- Understanding protein hydrogen bond formation
with kinetic H/D amide isotope effects
Click here for
more information
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